Generator

Part:BBa_K2012013:Design

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)


Strong CheZ generator with a fusion GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


Design Notes

N/A


Source

the CheZ coding sequence of this part is amplified from the wild type E.coli K12 MG1655 strain, the sequence is completely assistant with the sequence published on NCBI.

References