Generator
Part:BBa_K2012013:Design
Designed by: Wenqi Huang Group: iGEM16_HZAU-China (2016-10-06)
Strong CheZ generator with a fusion GFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1362
Design Notes
N/A
Source
the CheZ coding sequence of this part is amplified from the wild type E.coli K12 MG1655 strain, the sequence is completely assistant with the sequence published on NCBI.